None of these are an exact match, but Miss Muffet's (at about 3:09 in the first clip seems the best contender so far to me. His face is right, and he's not so mean. The one from Spider & the Fly, though, has the right gloves/shoes on his appendages (sometimes^^). Maybe the TDS one is an amalgamation? Anybody have a better answer?
Silly Symphony - Mother Goose Melodies - Little Miss Muffet
Silly Symphony - Spider & the Fly
[ame="http://www.youtube.com/watch?v=r8sSEDAUS_A"]1937 Short - The Worm Turns[/ame]
The spider here is not our guy, I think. I'm just including it cos I found it, and it has color and Mickey!^^