I would say yes, if you're talking about a second gate anywhere close to the current studio and theme park. Universal Hollywood is a major working film studio, they don't have a lot of extra space like a parking lot they can turn into another gate.
Just take a peek at this article for some idea of the opposition that Universal has encountered.
I heard the late Roy P. Disney was quite adamently opposed to bringing IoA's roller coasters to the other side of the LA River from his golf course and that legacy lives on. This article talks about other developments that Disney opposed at Universal, but it gives you an idea.